噬菌体Bp7穿孔素holin的克隆、表达及其活性分析

doi:10.11843/j.issn.0366-6964.2017.12.019

Abstract

Abstract:

To identify the sequence character and antibacterial activity of holin from phage Bp7, holin gene of phage Bp7 was amplified by PCR and amino acid sequence was compared and analyzed. Recombined plasmid pCold-holin was constructed and transferred into E. coli BL21 to express holin protein. The best induction conditions were explored. The protein was purified by affinity chromatography and its antibacterial activity was determined. The results showed that the holin of phage Bp7 contained a transmembrane region and belonged to Phage-holin-T family. The soluble protein was expressed in E. coli BL21. Turbidity test of E. coli BL21 showed that the expression of holin protein could significantly inhibit the proliferation of E. coli BL21, decrease the number of cells in 4 h, also the cells showed a agglutinative form. The results indicate that holin of phage Bp7 has antibacterial activity, which is helpful to further study of the antibacterial mechanism of holin.

CLC Number:

S852.6

QI Xin, CHEN Pei-pei, ZOU Ling, LIU Wen-hua, REN Hui-ying, ZHANG Can. Cloning, Expression and Activity Analysis of Phage Bp7 Holin[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(12): 2392-2398.

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Cloning, Expression and Activity Analysis of Phage Bp7 Holin

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